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. 2015 Jun 15;5(6):e163. doi: 10.1038/nutd.2015.13

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Copyright © 2015 Macmillan Publishers Limited

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Immunohistochemical analysis of hippocampal CA1 area. (a–f) Consistency of nestin (violet) and NeuN (green) staining in the CA1 hippocampal area of MGs. (a–c) Old control group. (d–f) 2-OX+PLEM group. (a and d) Nestin-positive cells (purple). (b and e) NeuN-positive cells (green), (c and f) merged. (g) Number of Nestin/NeuN-positive cells per 1 mm² of stratum pyramidale in hippocampal CA1 area. 2-OX-diet group—old MGs treated with 2-OX-enriched diet (n=14); PLEM-diet group—old MGs treated with PLEM-enriched diet (n=14); 2-OX+PLEM group—old MGs treated with both 2-OX and PLEM (n=17); old control group—control old MGs (n=18); young control group—young MGs (6 months; n=6). Data presented as mean±s.d. ^a,b,cMean values with unlike letters were significantly different between the groups (P⩽0.05).