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Specificity of the optimized multiplex assay in the detection of individual targets of CPPV (strain CCE41), CdPV (genotype CdPV1) and CMLV (strain CMLV-14) and unrelated DNAs extracted from SPV (sheeppox virus), LSDV (Lumpy skin disease virus) and BM (Brucella melitensis). PCR products were resolved by electrophoresis in 1.5 % agarose in tris-acetate EDTA (TAE) buffer (40 mM Tris–acetate pH 8.0,1 mM EDTA) and the gel was stained with ethidium bromide and photographed
