Table 2.
Testing clinical specimens to evaluate the performance of Monoplex and Multiplex PCR assays
| Country/area/year | No. of skin specimens | Suspected virus | Monoplex PCR positive specimens (%) | Multiplex PCR positive specimens (%) | Positivity by standard methods |
|---|---|---|---|---|---|
| Sudan/Showak/2000 | 6 | CMLV | 6 (100) | 6 (100) | ND* |
| Sudan/Showak/2013 | 8 | CMLV | 6 (75) | 6 (75) | 4 (50) |
| SaudiArabia/Hail/2014 | 3 | CMLV | 3 (100) | 3 (100) | 3 (100) |
| Sudan/Showak/1993 | 6 | CPPV | 4 (66.7) | 4 (66.7) | 3 (50) |
| Sudan/Showak/2000 | 8 | CPPV | 7 (87.5) | 7 (87.5) | ND |
| Sudan/Showak/2005 | 6 | CPPV | 6 (100) | 6 (100) | ND |
| Sudan/Showak/2012 | 6 | CPPV | 4 (66.7) | 4 (66.7) | ND |
| Sudan/Showak/2013 | 5 | CPPV | 3 (60) | 3 (60) | ND |
| Sudan/Showak/2013 | 8 | CPPV | 6 (75) | 6 (75) | ND |
| Saudi Arabia/Hail/2014 | 3 | CPPV | 0 | 0 | ND |
| Sudan/Showak/1994 | 5 | CdPV | 5 (100) | 5 (100) | ND |
| Sudan/Khartoum/2009 | 6 | CdPV | 5 (83.3) | 5 (83.3) | ND |
| Sudan/Showak/2012 | 4 | CdPV | 3 (75) | 3 (75) | ND |
| Sudan/Showak/2013 | 3 | CdPV | 3 (100) | 3 (100) | ND |
| Saudi Arabia/Al-Ahsa/2013 | 3 | CdPV | 0 | 0 | ND |
| Saudi Arabia/Al-Ahsa/2014 | 6 | CdPV | 4 (66.7) | 4 (66.7) | 4 |
| Total | 86 | 65 (75.6) | 65 (66.7) |
ND: not done