Figure 1.

Actin remodeling in sperm capacitation and prior to acrosome reaction: human spermatozoa were incubated under capacitation conditions in Ham's F-10 medium and after 3 h of incubation the Ca²⁺-ionophore A23187 was added (upper figure). At the indicated times cell samples were stained for F-actin using FITC-phalloidin, photographed under fluorescence microscope, and analysed for fluorescence intensity in the sperm cells (upper figure). A picture of the stained cells is seen in the lower figure.