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SCF in Epididymal and Vas Deferens Spermatozoa. These data were previously published in Yamauchi, et al.16 Spermatozoa from the epididymis (lanes 1–7) or the vas deferens (lanes 8–14) were embedded in agarose plugs, and incubated in mHCZB (modified Hepes-CZB, CZB medium buffered with HEPES) supplemented with MnCl2 and CaCl2 as described,16 for the times indicated, and treated with or without EDTA to reverse TOP2B-induced breaks. The plugs were then electrophoresed by FIGE.
