Fig. 5.

Down-regulation of DAXX represses HPV early gene expression. U2OS cells were first transfected with Daxx siRNA or a control siRNA, and 24 h later with wt HPV18 or wt HPV11 genome. 48 h after transfection total RNA was isolated, reverse transcribed and analyzed by qPCR. Each sample was analyzed in triplicate with E6, E7, E1, E2, beta-actin and HPRT1 specific primers. HPV11 and 18 (a) and bACT (b) mRNA levels were calculated relative to HPRT1 mRNA levels. The data represent the average of three independent experiments. Blow-out of HPV18 part from (a) is shown in (c). d The location of four different primer pairs targeted against HPV E6, E7, E1 and E2 coding sequences in HPV18 early region