Table 1.

BLAST analyses to Arabidopsis confirm a genome duplication event in M. sativa

	P. vulgaris	G. max	M. truncatula	M. sativa
Number of primary transcripts	27,197	56,044	50,894	112,626
Transcripts with BLASTX hit to Arabidopsis	25,119	49,304	35,644	64,631
Unique Arabidopsis sequences	14,283	15,407	15,170	15,607

The primary transcripts of Phaseolus vulgaris, Glycine max, Medicago truncatula, and Medicago sativa (MSGI 1.2) were compared to the 27,416 predicted primary proteins of Arabidopsis using BLASTX with an E-value cutoff of 1E-10. The ratio of transcripts from each legume species that match an Arabidopsis protein clearly reflects a whole genome duplication event in G. max relative to P. vulgaris and a polyploidy event in M. sativa relative to M. truncatula. A similar number of unique Arabidopsis sequences were identified from the BLAST report for each legume, confirming the breadth of the M. sativa assembly