Table 1.
Summary of treatment conditions on BV-2 and IMA2.1 cells and their effects on VACV infection
| Treatment condition | BV-2 | IMA2.1 | BV-2 | IMA2.1 |
|---|---|---|---|---|
| Virus progenya | VACV-mediated cell deathb | |||
| M2 phenotype | ||||
| w/o | + | 0 | + | − |
| IL-4 | + | nt | + | − |
| M1 phenotype | ||||
| IFN-γ | − | − | − | − |
| IFN-γ + LPS | − | − | − | − |
| LPS | − | − | − | − |
nt not tested, w/o without stimulation.
aVirus progeny (24 and 72 hpi) related to the infection medium was determined by standard plaque assays. [+] indicates an increase of the virus titer from the infection medium, [0] virus titer at the same level as the infection medium, [−] virus titer below the infection medium.
bVACV-mediated cell death was related to uninfected controls (24–72 hpi) and measured by MTT cell survival assays. [+] more than 50% of cells were killed by LIVP 1.1.1 [−] less than 50% of cells were killed by LIVP 1.1.1.