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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: J Mol Cell Cardiol. 2012 Aug 19;53(5):668–676. doi: 10.1016/j.yjmcc.2012.08.010

Fig. 3.

Fig. 3

Stability and distribution of urine miR-1 in rats with AMI. (A) Urine samples were collected from rats at 24 h after AMI and miR-1 levels were determined immediately after urine collection and 24 h later at 4 °C. n=5, *P<0.01 compared with 0 h group. (B) Representative urine exosomes isolated by ultracentrifugation and detected by electron microscopy. (C) The distribution of urine miR-1 in exosome part and supernatant part (100% of total urine miR-1). (D) miR-1 levels in supernatant part of urine in rats with and without AMI. (E) miR-1 levels in exosome part of urine in rats with and without AMI. (F) miR-1 levels in freshly isolated supernatant part of AMI urine and in supernatant part kept for 24 h at 4 °C. (G) miR-1 levels in freshly isolated exosome part of AMI urine and in exosome part kept for 24 h at 4 °C. Note: n=5, *P<0.01 compared with 0 h group.