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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: Biotechnol Prog. 2015 Mar 1;31(2):334–346. doi: 10.1002/btpr.2038

Figure 5. Two-color FACS analysis of SNA and MALII lectin staining of ST6GAL1 overexpression clones.

Figure 5

Representative scatter plots with quadrant analysis from the 12 clones overexpressing ST6GAL1 are shown here. The X-axis is fluorescence of FITC-SNA. The Y-axis is fluorescence of AlexaFluor647, representing MALII lectin staining. Each quadrant is labeled with % of total population. The gating was set based on streptavidin-AlexaFluor647 only of each OE clone (scatter plots not shown here) and based on FITC of the nontransfected host cell line, respectively, to exclude background fluorescence.