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. 2015 Jul 6;6:345. doi: 10.3389/fimmu.2015.00345

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Copyright © 2015 Kumari, Zhang, Swain, Chi, Niu, Bøgwald and Dalmo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree showing the relationship of salmon T-bet gene with other known vertebrate members of the Tbr1 subfamily. The phylogram was constructed on ClustalX2 and MEGA 4.1. The neighbor-joining (N-J) method with bootstrap values of 1000 replications was adopted. Accession numbers or ENSEMBL gene IDs are as follows: Human (Homo sapiens) Eomes, NP_005433; Mouse (Mus musculus) Eomes, NP_034266; Cattle (Bos taurus) Eomes, NP_001178117; Frog (Xenopus laevis) Eomes, NP_001081810; Chicken (Gallus gallus), Eomes, XP_426003; Zebrafish (Danio rerio) Eomes a, NP_571754; Zebrafish Eomes b, NP_001077044; Salmon (Salmo salar) Eomes, EU418014; Amphioxus (Branchiostoma floridae) Eomes/Tbr1/Tbx21, AF262568; Human Tbr1, NP_006584; Mouse Tbr1, NP_033348.2; Cattle Tbr1, NP_001178978; Zebrafish Tbr1, NP_001108562; Human T-bet, NP_037483; Mouse T-bet, NP_062380; Cattle T-bet, NP_001179069; Pig (Sus scrofa) T-bet, ENSSSCP00000018565; Frog T-bet, NP_001088247; Lizard (Anolis carolinensis) T-bet, ENSACAP00000006911; Medaka (Oryzias latipes) T-bet, ENSORLP00000015259; Fugu (Takifugu rubripes) T-bet, ENSTRUP00000032964; Stickleback (Gasterosteus aculeatus) T-bet, ENSGACP00000005023; Tetraodon (Tetraodon nigroviridis) T-bet, ENSTNIP00000013001; Zebrafish T-bet, NP_001164070; Crucian carp (Carassius auratus langsdorfii) T-bet, BAF73805.1; Trout (Oncorhynchus mykiss) T-bet, NM_001182722.