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. 2015 Jul 6;6:345. doi: 10.3389/fimmu.2015.00345

Figure 6.

Figure 6

Regulators of T-bet expression in salmon leukocytes. Spleen leukocytes were stimulated for 24, 48, and 72 h with ConA + PHA + huIL-2, IFN-α (0.5 mg/ml and 5 ng/ml), and the mRNA levels of T-bet were determined by real-time PCR. Gene expression is normalized against EF-1α and is shown relative to the mean of the non-stimulated cells (leukocyte cells with no treatment, i.e., only media control). Spleen leukocytes with no treatment were considered as control for each time points. Each bar represents the mean ± SE of triplicate samples. Different letters denote statistically significant differences (P < 0.05) between the groups.