Table 1.

List of primers and their designated applications.

Primer name	Sequence (5′–3′)	Application	GenBank accession number
AsTbet-F	CCATCGCTGCAGGACAAGCCCAAG	3′RACE/cDNA cloning
AsTbet-NestF	CGGTCAAATCGGTGGACTCTGCTG	3′RACE
AsTbet-R	CGCCCTCAAACAGGCCGGAGTC	5′RACE/cDNA cloning
AsTbet-NestR	CCTTGGGCTTGTCCTGCAGCGATG	5′RACE
AsTbet-F	GTACCACGCAGACCAAACTG	5′RACE extension
AsTbet-R	GGGCAGAGGGTTCATGTAC	5′RACE extension
M13 F	CAGGAAACAGCTATGAC	Sequencing
M13 R	GTAAAACGACGGCCAG	Sequencing
T3 F	ATTAACCCTCACTAAAGGGA	Sequencing
T7 R	TAATACGACTCACTATAGGG	Sequencing
AsTbet-F	GGCATAGGTGGCAATCTTTACC	Real-time PCR	GU979861
AsTbet-R2	GTGCCGATCCGCCCTGTC	Real-time PCR
AsNKEF-F	TGCCGAGGAGTTTAGGAAGA	Real-time PCR	NM_001141386
AsNKEF-R	AATCTTCATGGCACCCAGAC	Real-time PCR
AsIL22-F	GGCCCGAGTCAGCAGAGACCT	Real-time PCR	DW572073
AsIL22-R	CTCCTCCATCCCGGCCAACTTC	Real-time PCR
AsIFNγ-F	CGTGTATCGGAGTATCTTCAACCA	Real-time PCR	NM_001123558
AsIFNγ-R	CTCCTGAACCTTCCCCTTGAC	Real-time PCR
AsEFα-F	CGGCAAGTCCACCACCAC	Real-time PCR	AF308735
AsEFα-R	GTAGTACCTGCCAGTCTCAAAC	Real-time PCR