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. 2015 Apr 2;126(1):80–88. doi: 10.1182/blood-2014-07-587600

Figure 3.

Figure 3

FlnA potentiates PACSIN2 F-BAR domain membrane tubulation in vitro. (A) PC/PS liposomes were incubated with GST-PACSIN2 F-BAR domain WT or P180A in the presence or absence of His-FlnA constructs, as indicated. Membrane tubulation capacity was assessed in the electron microscope. (B) Diameter of membrane tubules generated by GST-PACSIN2 (P2) F-BAR domain in the presence or absence of His-FlnA. (C) PC/PI liposomes were incubated with GST-PACSIN2 (P2) F-BAR domain and/or EGFP-FlnA and observed by light and fluorescence microscopy.