Figure 5.
Design and operation of cartridge purification module (CPM). (a) Schematic of fluid path. Reagent reservoirs are connected to a common path leading through the cartridge to waste and output valves. Remaining panels show operating states: (b) User loads reagents through check-valves into reservoirs during setup prior to synthesis: (i) diluent solution to dilute sample prior to passing through cartridge, (ii) wash solution to wash cartridge after trapping, and (iii) eluent solution to elute from cartridge to output. (c) Sample is loaded via a tube connected to the diluents loading port. It is flowed into the diluent solution and followed by nitrogen bubbling to induce mixing. (d) Diluted sample is flowed through the cartridge to trap the desired species. (e) Cartridge is washed by flowing wash solution through to waste. (f) Purified compound is eluted from cartridge with eluent solution to output.