Skip to main content
. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: Science. 2015 May 22;348(6237):917–921. doi: 10.1126/science.aaa9266

Figure 3.

Figure 3

Three-dimensional structure of ΨCES. (A) Ensemble of 20 refined structures (residues 105–344 shown). (B and C) Expanded views of the (B) three-way-1 and (C) three-way-2 junctions. (D) Surface representation of ΨCES highlighting U5 (blue):AUG (green) base pairing and the integral participation of SD residues (pink) in the tandem three-way junction structure. (E) Severe line broadening indicative of slow (millisecond) conformational averaging was observed for stacked, mismatched pyrimidines in the extended DIS stem (yellow in (D); broadened C287-H1′ signal boxed in (E)). NOE patterns and sharp NMR signals also indicate that the ψ hairpin loop is unstructured (red in (D)).