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The localization of ZTF-8 is altered in SUMOylation-defective mutants but not in non-SUMOylatable ZTF-8 mutants. Immunolocalization of ZTF-8 in indicated genotypes of SUMOylation pathway-deficient mutants and non-SUMOylatable ZTF-8 transgenic worms: 1KR ztf-8(rj24), 3KR ztf-8(rj25), and 4KR ztf-8(rj26). Costaining of nuclei with DAPI (blue) and an anti-ZTF-8 antibody (green). Images show premeiotic tip, transition zone (leptotene/zygotene stages), and late pachytene nuclei. Bar, 2μm.
