Figure 5.

Reporter gene expression in larval tissues at two different rearing temperatures. mRNA abundance was measured via RT-qPCR in brains (A and B) and midguts (C and D) from third instar larvae reared at either 17° (shaded bars) or 28° (open bars). In A and C the ph-p promoter is driving lacZ expression whereas in B and D lacZ is under the control of the CG3835 promoter. The intergenic region containing the PRE and the promoters was taken from either a European strain from The Netherlands (NL) or an African one from Zimbabwe (ZK); that is, constructs were carrying either the derived or the ancestral sequence variants, respectively. Error bars represent the 95% confidence interval. *P < 0.05, **P < 0.01 (FDR = 0.05).