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. 2015 Apr 24;24(15):4185–4197. doi: 10.1093/hmg/ddv151

Figure 2.

Figure 2.

The acetyl-transferase domain of p300 is required to induce ΔNp63α protein stabilization. (A) WB analysis of whole HaCaT cell extracts transiently transfected with increasing amounts (20 ng and 40 ng) of shp300 or shLuc expression vectors (B) WB analysis of HaCaT whole cell extracts transiently co-transfected with equal amount of ΔNp63α expression vectors (30 ng) and increasing amounts of p300 encoding plasmids (p300 (WT) or p300-LY-RR, mutated in the HAT domain (10 and 20 ng). (C) WB analysis of U2OS whole cell extracts transiently co-transfected with equal amount of ΔNp63α expression vectors (30 ng) and increasing amounts of p300 expression vectors (10 and 20 ng).