Figure 2.

Altering UPS function modulates RAN translation-elicited inclusion number in CGG-repeat-expressing Drosophila. (A) Schematic of the CGG-repeat transgene. Both FMRpolyG-GFP (via RAN translation) and GFP (via AUG initiated translation) are generated in flies expressing this transcript. Red is human-derived sequence. (B) Flies expressing CGG₁₀₀GFP, but not GFP alone, produce large GFP-positive aggregates in the ommatidia when crossed to gmr-GAL4. (C) Representative transverse sections through fixed and permeabilized Drosophila eyes of the indicated genotypes. (D) Quantitation of the number of GFP-positive inclusions in flies expressing CGG₁₀₀GFP alone or with DTS7 or HSP70. *P < 0.05 on Student's t-test compared with gmr-Gal4; CGG₁₀₀GFP alone.