Figure 3.

RAN translation is required for modulation of CGG-repeat-mediated toxicity by UPS impairment. (A) Top row: representative images of indicated genotypes. StopCGG₁₀₀ includes a stop codon just before the repeat that precludes translation of FMRpolyG. ATGCGG₁₀₀ includes a start codon just before the repeat that enhances FMRpolyG translation. Bottom row: same lines crossed to uas-DTS7, which impairs the proteasome. (B) Quantitation of eye phenotypes from flies expressing the indicated genotypes at 28°C. *P < 0.05 on Mann–Whitney U-test compared with DTS7 alone. # indicates significantly different from DTS7 × CGG₁₀₀ and DTS7 × ATGCGG₁₀₀.