Skip to main content
. 2015 May 7;24(15):4340–4352. doi: 10.1093/hmg/ddv167

Figure 6.

Figure 6.

siRNA and qRT-PCR in HeLa cells show that reduced DLX4 expression results in up-regulation of BMP4. Gene-specific primers are indicated on the X-axis; relative mRNA expression is indicated on the Y-axis. The first columns show data from untreated wells; expression for these wells has been normalized to 1 for comparison. The second columns show data from wells treated with 60 nm DLX4 siRNA treatment and the third columns show data from wells treated with 60 nm GAPDH siRNA. The first set of three columns confirms reduced expression of DLX4 after 60 nm DLX4 siRNA treatment (second column) using DLX4-secific primers. Similarly, the second set of columns confirms reduced expression of GAPDH after 60 nm GAPDH siRNA treatment (third column) using GAPDH-specific primers. The third and fourth sets of columns show increased BMP4 expression after 60 nm DLX4 siRNA treatment (second columns) using two primer sets for BMP4. The remaining sets of columns show no significant changes in BMP2, BMP5, Noggin and BMPER after 60 nm DLX4 siRNA treatment (second columns). All results are expressed as the mean ± standard error of the mean; sample size n = 3.