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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: Biochemistry. 2015 Apr 29;54(18):2858–2873. doi: 10.1021/acs.biochem.5b00064

Table 3. Circular Dichroism and Thermal Denaturation Parametersa.

sample −[θ]222 nm (deg cm2 dmol−1 res−1) [θ]222 nm/[θ]208 nm Tm (°C) ΔHcalb (kcal mol−1) ΔHcalHvan't Hoff
α3Dc 25213 (306) 1.01 89.6 (0.3) 49.9 (4.6) 1.2 (0.3)
α3DIV 29231 (672)c 1.03 64.4 (0.6)d 50.0 (0.2) 2.4 (0.4)
60.2 (0.1)c 44.9 (1.2) 1.9 (0.2)
Hg-α3DIVc 27589 (421) 1.51 84.0 (1.7) 65.1 (3.5) 6.7 (2.4)
Pb-α3DIVc 26940 (2420) 1.01 83.4 (3.3) 59.2 (6.9) 4.5 (2.3)
Cd-α3DIVc 27600 (487) 1.07 78.1 (0.7) 52.7 (4.3) 4.2 (0.5)
a

All the averaged values were determined from triplicate or duplicate experiments.

b

Calculated excess heat capacity.

c

pH 8.2.

d

pH 7.0.