Table 1.
Primer sequences.
| Gene | Rat | Non-human primate |
|---|---|---|
|
MT-MMP1 (MMP-14) |
Forward sequence 5′-AAAGGGAACAAATACTGGAA-3′ | Forward sequence 5′- CCACCTACGGACCCAACATC-3′ |
| Reverse sequence 5′-ATGTAGTTAGGGGGATGGAA-3′ | Reverse sequence 5′- CAGAACCAGCGCTCCTTGAA | |
| MMP-2 | Forward sequence 5′-CAATACCTGAACCTT-3′ | Forward sequence 5′- ATGTTGTCTTGTGAGCGTGC-3′ |
| Reverse sequence 5′-CTGTATTGATCTGGTT-3′ | Reverse sequence 5′- AGGTATTGGCAACACTGCGG-3′ | |
| TIMP-2 | Forward sequence 5′-ATTTATCTACACGGCCCC-3′ | Forward sequence 5′- GGGCTGCGAGTGTAAGATCA-3′ |
| Reverse sequence 5′-CAAGAACCATCACTTCTCTTG-3′ | Reverse sequence 5′- AAGAAACTCCTGCTTGGGGG-3′ | |
| ICAM-1 | Forward sequence 5′-AGCATTTACCCCTCACCCAC-3′ | Forward sequence 5′- CAAACCTTTGACCTGCCAGC-3′ |
| Reverse sequence 5′-CATTTTCTCCCAGGCATTCTC-3′ | Reverse sequence 5′- CGAGAGGGAGTTGTTGCCAT-3′ | |
| CDH-2 | Forward sequence 5′-CACCCGGCTTAAGGGTGATT-3′ | Forward Sequence 5′- GGAAAAGTGGCAAGTGGCAG-3′ |
| Reverse sequence 5′-CGATCCTGTCTACGTCGGTG-3′, | Reverse sequence 5′- GTGGCTCCTTCACTGACTCC-3′ | |
| ITG- α5 | Forward sequence 5′-GAAGGGACGGAGTCAGTGTG-3′ | Forward sequence 5′- GGGTACCTGCTACCTCTCCA-3′ |
| Reverse sequence 5′-CTGGGTCATTCTGTGGGTCC-3′, | Reverse sequence 5′- GCTGAAATCTGAGCGGCAAG-3′ | |
| ITG- β1 | Forward sequence 5′-TTCAGACTTCCGCATTGGCT-3′ | Forward sequence 5′- GCCAAATGGGACACAGGTGA-3′ |
| Reverse sequence 5′-CCAATCAGCGACCCACAAAC-3′ | Reverse sequence 5′- TGCACAGGCGGTACTCATTT-3′ | |
| Collagen I | Forward sequence 5′-GGTTCTCCTGGCAAAGATGGACT-3′ | Forward sequence 5′- GTTTCTCCTTGGGGTCGGAG-3′ |
| Reverse sequence 5′-ACTGGTCATGCTCTCTCCAAACCA-3′, | Reverse sequence 5′- TGGTGGGATGTCTTCGTCTTG-3′ | |
| Collagen III | Forward sequence 5′-TCCTAACCAAGGCTGCAAGATGGA-3′ | Forward sequence 5′- AATCAGGTAGACCCGGACGA-3′ |
| Reverse sequence 5′-AGGCCAGCTGTACATCAAGGACAT-3′, | Reverse sequence 5′- TTCGTCCATCGAAGCCTCTG-3 | |
| Lama 2 | Forward sequence 5′-GCCACACGAGACCTGAAAGA-3′ | Forward sequence 5′- CAGATAGCGTCGCCAAAACG-3′ |
| Reverse sequence 5′-ACAAAACCAGGCTTGGGGAA-3′ | Reverse sequence 5′- AATGCAGTCACCTCCCGAAG-3′ | |
| Lama 4 | Forward sequence 5′-GCCACACGAGACCTGAAAGA-3′ | Forward sequence 5′- ACATTGAAGGGAGCTCAGCG-3′ |
| Reverse sequence 5′-ACAAAACCAGGCTTGGGGAA-3′ | Reverse sequence 5′- CATTGCATTTCTCGGCAGCA-3′ | |
| B-Actin | Forward sequence 5′-CGCCACCAGTTCGCCATGGAT-3′ | Forward sequence 5′-AGGAGAAGCTGTGCTACGTC-3′ |
| Reverse sequence 5′-TAGGGCGGCCCACGATGGAG-3′ | Reverse sequence 5′-ACTCCATGCCCAGGAAGGAA-3′ |