Figure 3.20.3.

Sequence information for primers. Kozak sequences (CACC) were placed just in front of ATG sequences. In the Gateway® scheme, the partial att sequences are underlined in the case of gene-specific primers. In the In-Fusion® scheme, underline sequence highlight the 15 bases of homology to the entry vector. Shown is homology required to clone into Gateway® -compatible entry vector. To obtain C-terminal fusion proteins in the case of open primers, the common stop anticodon (CTA) is changed to the leucine anticodon (CAA). For the Gateway® scheme only, the universal primers contain complete att sequences and the matching sequences to gene-specific primers are underlined.