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. 2015 Jul 6;9(7):e0003920. doi: 10.1371/journal.pntd.0003920

Fig 3. SmLy6A and SmLy6B are both abundantly expressed in adult schistosomes but only SmLy6B is detectable in schistosomula.

Fig 3

SmLy6A and SmLy6B mRNA and protein abundances were elucidated as described in the Materials and Methods. Transcription profiles of SmLy6A (A) and SmLy6B (B) were derived from the S. mansoni lifecycle microarray data available via Array express [10] under the experimental accession number E-MEXP-2094. Values are mean normalized fluorescence units ± sem (standard error of the mean). Recombinant SmLy6A (C) and SmLy6B (D) were cloned into a modified pET30a vector (Novagen), expressed using BL21 star cell line (Invitrogen) and purified from inclusion bodies using Ni-NTA affinity columns (Qiagen). (E) Anti-rSmLy6A polyclonal rat antisera recognises a ~10kDa native protein found within soluble extracts derived from mixed-sex 7-week old worms. (F) Anti-rSmLy6A polyclonal rat antisera recognises a ~10kDa native SmLy6B from 24 schistosomula and 7 week worms. Pre-bleed rat serum did not recognise either protein.