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Systematic analysis of buffer effects on qPCR quantitation of plasmid DNA. (A) qPCR results of 108 to 101 copies of vector plasmid DNA performed in (1) H2O (cross-hatched columns), (2) DT buffer only (open columns), (3) buffer/inactivated DT (hatched columns), and (4) buffer/inactivated DT/PK/heat at 95°C (solid columns); (B) calibration curves generated from qPCR of plasmid DNA in various buffers, showing data linear range, R2 and amplification E values (n=3).
