Fig. 5.
Comparison of amplification background signals obtained from PBS control samples after treatment by method A or method B and calculated on the basis of circular [(A), (C), and (E)] or linear [(B), (D), and (F)] plasmid DNA, using ITR primers (A and B), GFP primers (C and D), or CMV primers (E and F). Dashed lines indicate the set Cq cutoff value and a valid detection value, and potentially the cross-contamination of AAV DNA sequence during sample preparation when the copy number is above the dashed lines (n=3). ns, not significantly different.