Fig. 6.

Comparison of AAV genome titers in samples prepared by method A (hatched columns) or method B (gray columns). Vector titers (VG/ml) were calculated on the basis of circular plasmid [(A), (C), and (E)] or linear plasmid [(B), (D), and (F)], using ITR primers (A and B), GFP primers (C and D), or CMV primers (E and F). Data shown represent mean data of nine PCRs of the same sample extracted in triplicates and then qPCR in triplicate (n=9). Asterisks indicate statistical significance observed between the two methods, where *p<0.05, **p<0.01, ***p<0.001.