Table 1.
Target | ID | Sequence (5′→3′)a | Tm (°C) | Amplicon (bp) |
---|---|---|---|---|
CMV promoter | CMV-F | TTC CTA CTT GGC AGT ACA TCT ACG | 70 | 129 |
CMV-R | GTC AAT GGG GTG GAG ACT TGG | 66 | ||
CMV-P | TGA GTC AAA CCG CTA TCC ACG CCC A | 78 | ||
AAV2 ITRb | ITR-F | GGA ACC CCT AGT GAT GGA GTT | 64 | 62 |
ITR-R | CGG CCT CAG TGA GCG A | 54 | ||
ITR-P | CAC TCC CTC TCT GCG CGC TCG | 72 | ||
Humanized Renilla green fluorescent proteinc | hrGFP-F | GAT CCG CAG CGA CAT CAA CC | 64 | 136 |
hrGFP-R | GTA CAC CAC CTC GAA GCT GG | 64 | ||
hrGFP-P | GAG GAG ATG TTC GTG TAC CGC GTG G | 80 | ||
SV40 polyadenylation signal | SV40-F | AGC AAT AGC ATC ACA AAT TTC ACA A | 70 | 96 |
SV40-R | CCA GAC ATG ATA AGA TAC ATT GA | 62 | ||
SV40-P | AGC ATT TTT TTC ACT GCA TTC TAG TTG TGG TTT GTC | 98 |
AAV2, adeno-associated virus serotype 2; CMV, cytomegalovirus; F, forward; ITR, inverted terminal repeat; P, probe; R, reverse; SV40, simian virus 40; Tm, annealing temperature.
Hydrolysis probes were dual-labeled with 6-FAM at the 5′ end and TAMRA at the 3′ end.
From Aurnhammer and colleagues.16
Designed to EF186083.1.