Table 1.

Real-Time Quantitative PCR Primer and Probe Sequences

Target	ID	Sequence (5′→3′)a	Tm (°C)	Amplicon (bp)
CMV promoter	CMV-F	TTC CTA CTT GGC AGT ACA TCT ACG	70	129
	CMV-R	GTC AAT GGG GTG GAG ACT TGG	66
	CMV-P	TGA GTC AAA CCG CTA TCC ACG CCC A	78
AAV2 ITRb	ITR-F	GGA ACC CCT AGT GAT GGA GTT	64	62
	ITR-R	CGG CCT CAG TGA GCG A	54
	ITR-P	CAC TCC CTC TCT GCG CGC TCG	72
Humanized Renilla green fluorescent proteinc	hrGFP-F	GAT CCG CAG CGA CAT CAA CC	64	136
	hrGFP-R	GTA CAC CAC CTC GAA GCT GG	64
	hrGFP-P	GAG GAG ATG TTC GTG TAC CGC GTG G	80
SV40 polyadenylation signal	SV40-F	AGC AAT AGC ATC ACA AAT TTC ACA A	70	96
	SV40-R	CCA GAC ATG ATA AGA TAC ATT GA	62
	SV40-P	AGC ATT TTT TTC ACT GCA TTC TAG TTG TGG TTT GTC	98

AAV2, adeno-associated virus serotype 2; CMV, cytomegalovirus; F, forward; ITR, inverted terminal repeat; P, probe; R, reverse; SV40, simian virus 40; T_m, annealing temperature.

^aHydrolysis probes were dual-labeled with 6-FAM at the 5′ end and TAMRA at the 3′ end.

^bFrom Aurnhammer and colleagues.¹⁶

^cDesigned to EF186083.1.