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. 2015 Jul 6;10(7):e0132009. doi: 10.1371/journal.pone.0132009

Fig 1. Dual-culture assay for bioactivity of volatile organic compounds produced by Ceratocystis fimbriata.

Fig 1

(a) Experimental design used with the following test organisms. Plate with C. fimbriata was on the bottom and test pathogen culture was on the top plate with the culture facing down. (b) CK: Monilinia fructicola growing on a PDA plate for 6 days. T: M. fructicola growing on a PDA plate with 6-day exposure to the VOCs from C. fimbriata. (c) CK: M. fructicola growing on a PDA plate for 4 days. R: M. fructicola after 6-day exposure to the VOCs from C. fimbriata transferred to a fresh PDA plate and grown for another 4 days. (d) M. fructicola, exposed to 0, 1, 3, 5 and 7-day old cultures of C. fimbriata for 5 days, respectively. (e) CK: Penicillium digitatum growing on a PDA plate for 6 days. T: P. digitatum growing on a PDA plate with 6-day exposure to the VOCs from C. fimbriata. (f) CK: P. digitatum growing on a PDA plate for 4 days. R: P. digitatum after 6-day exposure to the VOCs from C. fimbriata transferred to a fresh PDA plate and grown for another 4 days. (g) CK: P. italicum growing on a PDA plate for 5 days. T: P. italicum growing on a PDA plate with 5-day exposure to the VOCs from C. fimbriata. (h) CK: Phytophthora capsici growing on a CA plate for 6 days. T: P. capsici growing on a CA plate with 6-day exposure to the VOCs from C. fimbriata. (i) CK: Acidovorax avena growing on a LB plate for 2 days. T: A. avena growing on a LB plate with 2-day exposure to the VOCs from C. fimbriata.