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. 2015 Jul 6;10(7):e0131887. doi: 10.1371/journal.pone.0131887

Fig 1. Sensitivity test of the real-time PCR assay for the identification of T. urticae.

Fig 1

Plasmid containing ITS1 insert of T. urticae were series diluted to create calibration curves for sensitivity calculations. The standard curve built from Cq values against the log copy number (range = 107–10 copies) of ITS1 insert (n = 3). The 95% confidence intervals of the slopes were plotted with a blue line for LPMQ66 and a red line for LPMQ25. The r 2 = 0.993 for LPMQ66 and 0.990 for LPMQ25 were obtained for the assay.