Table 4. Repeatability and reproducibility of the real-time PCR assay.

Sample ID	DNA Ext. methods a	Repeatability b (%CV)		Reproducibility c (%CV)
		Run 1	Run 2	Run 1 ~ Run 2
Q10	QG	1.18	0.16	1.10
MZ5	ZR	0.29	0.74	0.96
Q25	QG	0.42	1.61	1.36
MQ54	QG	1.33	0.30	2.02
MK5	KF	0.30	0.52	0.36

Note:

^a all the DNA samples were extracted with one individual mite with different extraction methods, QG = Qiagen DNeasy Blood and Tissue kit; ZR = ZR Tissue & Insect DNA kit (Zymo Research, CA, USA); KF = Kingfisher Cell and Tissue kit (Thermo Scientific, USA).

^b Repeatability is calculated as the percent coefficient of variance (%CV) of Cqs of a sample within a single run.

^c Reproducibility is calculated as the percent coefficient of variance of Cqs of a sample across independent runs.