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. 2015 Jun 26;10:4185–4201. doi: 10.2147/IJN.S82714

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© 2015 Friedrich et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Concentration measurements of cellular SPIONs.

Notes: (A–C) HUVECs were cultured for 48 hours in medium containing 0–100 µg_Fe/mL SPIONs. The measured cellular SPION concentration is given in pg_Fe/cell and µg_Fe/mL cell lysate and is correlated to the SPION concentration in medium indicated as µg_Fe/mL medium and µg_Fe/cm² cell culture plate surface. Cellular SPION concentrations were measured with (A) UVS, (B) MPS, and (C) AAS. The columns present data acquired with SEON^LA (left panel), SEON^LA-BSA (middle panel), and Rienso^® (right panel). Data are expressed as the mean ± standard error (n=3 with technical triplicates). R² represents the coefficient of determination.

Abbreviations: SPION, superparamagnetic iron oxide nanoparticle; UVS, ultraviolet spectrophotometry; MPS, magnetic particle spectroscopy; AAS, atomic adsorption spectroscopy; SEON^LA, lauric acid-coated nanoparticles; SEON^LA-BSA, lauric acid/albumin bovine serum hybrid-coated nanoparticles; HUVECs, human umbilical vein endothelial cells.