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. 2015 Jun 26;9:3279–3292. doi: 10.2147/DDDT.S70993

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© 2015 Hraiech et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

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Whole-body inhalation exposure system.

Notes: The Glas-Col^® aerosol exposure chamber system (Glas-Col, Terre Haute, IN, USA) is presented here. The bacterial suspension at a known concentration (A) is placed into a glass vial that is a venturi nebulizer (B), connected to a leak-proof gas flow system (C). Pumps generate compressed gas flow to disperse the suspension into a fine mist. Pumps’ flow rate is adjusted on manual flowmeters (D). Durations of the aerosolization and decontamination time are set via digital interface software displayed on a screen window (E). High-efficiency particulate arrestance filters, gas incinerators, and UV decontamination (F) processes are all included in a whole block. The system operates under negative pressure. Before aerosolization starts, animals are placed in a stainless steel basket (G).