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. 2015 Jul 10;23(2):148–162. doi: 10.1089/ars.2014.6151

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Copyright 2015, Mary Ann Liebert, Inc.

© Jayne Louise Wilson et al. 2015; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 2. — CO retention and access of CORM-3 to the cell interior is dependent on heme. (A) Myoglobin (10 μM) and CORM-3 (8 μM) were added to buffer only (circles), wild-type (squares), or hemA mutant (triangles) cells in the presence of Na dithionite. The concentration of Mb-CO accumulated was measured at several time points in CO difference spectra. Data are plotted as means±SEM from ≥5 replicates. (B) CO-reduced minus reduced spectra of myoglobin (10 μM) and CORM-3 (8 μM) added to buffer only (solid line), wild-type (dotted line), or hemA mutant (dashed line) cells in the presence of Na dithionite at t=5 min. (C, D) Intracellular ruthenium levels in hemA (closed circles) and wild-type (open circles) cells were measured by ICP-AES over 120 min after exposure of cultures to 100 μM CORM-3 under anaerobic (C) or aerobic (D) conditions. Data are plotted as means±SEM from ≥3 biological replicates.