FIG. 2.

Western blot analysis of antigen specificities of selected monoclonal antibodies. Lane 1 is incubated with 1D7-H11; lane 2, 3A6-E4; lane 3, 3G6-F9. Among all hybridomas, only 1D7-H11, 3A6-E4, and 3G6-F9 can react with purified Toxoplasma gondii protein of 85 kDa, predicted as TgVP1, and 76 kDa as PfVP1 and PbVP1. All three hybridoma supernatants can detect another protein of approximately 50 kDa within purified T. gondii total protein. None of the three reacted with purified OFTu total protein.