FIG. 5.

Low-power photomicrographs of immunofluorescent multiple staining performed on the pulmonary ligament (fluorescence microscope). This tissue section is one of the serial sections following Figure 4C. (A) The green-colored cells indicate mesothelial cells that react with low-molecular weight cytokeratin CAM5.2 antibody. (B) The orange-colored cells indicate mesothelial cells and lymphatic endothelial cells that react with D2-40 antibody. (C) The yellow- or green-colored cells represent mesothelial cells, and the orange-colored cells represent lymphatic endothelial cells. Asterisks indicate submesothelial lymphatic lumens. The scale bars indicate 20 μm. A color version of this figure is available in the online article at www.liebertpub.com/lrb