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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: J Biol Chem. 2001 Aug 16;276(42):38652–38657. doi: 10.1074/jbc.M106422200

Fig. 1. Photo-cross-linking of rTFIIA on the AdMLP.

Fig. 1

A, photo-cross-linking experiments with photoprobes −39/−40 and +26 were performed in the presence of rTFIIA (α/β and γ), TBP, TFIIB, TFIIF (RAP74 and RAP30), TFIIE (p56 and p34), and RNAPII. The specificity of the cross-linking signals was assessed by comparing reactions performed with all the factors to ones performed in the absence of TBP. Under our reaction conditions, the absence of TBP has the same effect as the use of a photoprobe with a mutation in the TATA element (TATAAAA to TAGAGAA) (55). The position of TFIIA α/β and TFIIA γ are indicated. B, schematic representation of promoter contacts by TFIIA in the context of the TBP-TFIIA-promoter complex and the TBP-TFIIA-TFIIB-TFIIF-RNAPII-TFIIE-promoter complex in which the DNA adopts a wrapped structure. Positions −39/−40, +26 and +1 are indicated. Only TBP, TFIIA, and RNAPII are represented to simplify the diagram.