Figure 3.

Tomato concentrate containing the 6F peptide (Tg6F), but not control tomato concentrate (EV) reduced metastasis of colon cancer cells to the lungs. Female BALB/c mice 6 weeks of age (n = 12 per group) were injected with 2 × 10⁴ CT26 cells in 100 μL of PBS via tail vein as described in Materials and Methods. After injection, the mice were maintained on either standard mouse chow (Chow), or standard mouse chow containing 0.06% by weight of the control transgenic tomato concentrate (EV), or standard mouse chow containing 0.06% by weight of the transgenic 6F tomato concentrate (Tg6F), which provided the mice with a dose of tomato concentrate of ∼120 mg/kg per day per mouse, which provided the mice with a dose of the 6F peptide of ∼7 mg/kg per day. After 4 weeks the mice were subjected to a terminal bleed, and after sacrifice the lungs were harvested, weighed, and fixed with Bouin’s solution, and the number of tumor nodules on the surface of the lungs was determined as described in Materials and Methods. (A) Weight of the lungs in grams. (B) Number of tumor nodules on the surface of the lungs. (C) Plasma levels of lysophosphatidic acid 20:4 (LPA 20:4). The data shown are mean ± SEM; NS, not significant. These results are representative of two of two experiments.