Figure 5.

Both the control tomato concentrate (EV) and the tomato concentrate containing the 6F peptide (Tg6F) significantly reduced ovarian cancer cell tumor burden, but the tomato concentrate containing the 6F peptide was significantly more effective. Female C57BL/6J mice at age 9 weeks (n = 24 per group) were given an intraperitoneal injection containing 8 × 10⁶ ID8 cells in a total volume of 0.8 mL of DMEM (without supplements). Following injection of the ID8 cells the mice were maintained on either standard mouse chow (Chow), or standard mouse chow containing 0.06% by weight of the control transgenic tomato concentrate (EV), or containing 0.06% by weight of the transgenic 6F tomato concentrate (Tg6F), which provided the mice with a dose of the 6F peptide of ∼7 mg/kg per day. After 12 weeks the mice were subjected to a terminal bleed, and after sacrifice the number of tumor nodules on peritoneal surfaces and on the surface of abdominal organs was determined as described in Materials and Methods. (A) The total number of tumor nodules in the abdomen. (B) The number of tumor nodules on the surface of the peritoneal cavity. (C) Number of tumor nodules on the diaphragm. (D) The number of tumor nodules on the surface of the intestine. (E) The number of tumor nodules in the abdomen that were greater than 1 mm in size. The data shown are mean ± SEM; NS, not significant. These results are representative of two of two experiments.