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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: J Biol Chem. 2001 Dec 31;277(11):9302–9306. doi: 10.1074/jbc.M110516200

Fig. 5. Efficient formation of a U2-dependent complex by splicing factors associated with the RNA Pol II-containing complex.

Fig. 5

Nuclear extracts (lanes 2–5) and the RNA Pol II-containing complex (Holo; lanes 10–13) were incubated with an adenovirus pre-mRNA containing a splicing cassette. A nuclear extract (NEΔU2; lanes 6–9) and the RNA Pol II-containing complex (HoloΔU2; lanes 14–17) depleted of snRNA U2 were used as controls. Splicing complex formation was monitored over time as indicated. The positions of the H and A complexes formed in nuclear extracts are indicated. The RNA Pol II-containing complex also leads to the assembly of a U2-dependent complex (RP) that nearly migrates with the A complex.