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. Author manuscript; available in PMC: 2015 Jul 6.
Published in final edited form as: J Alzheimers Dis. 2006 Dec;10(4):379–390. doi: 10.3233/jad-2006-10406

Figure 4.

Figure 4

Representative Western Blots comparing the labelling of AβPP in MOG and SY5Y cells obtained with (A) 2B12 and (B) a commercial anti-N-terminus AβPP antibody (Sigma-Aldrich). 50μg cell lysate samples were separated on a 10% SDS-PAGE gel, transferred to nitrocellulose membranes and probed with either 2B12 (2.7 μg/ml) followed by an anti-mouse IgG-HRP conjugate (1:30,000) or the n AβPP antibody (1:1000) followed by an anti-rabbit IgG-HRP conjugate (1:20,000) and the signal was detected using ECL. A 106kDa band corresponding to AβPP 695 and a 56kDa band corresponding to a thrombin cleavage product of AβPP were seen in both cell lines with both antibodies. n=3-6.