Figure 5.

Effect of NOSH-aspirin (NOSH-ASA) and aspirin on carrageenan-induced neutrophil migration and local production of pronociceptive cytokines. Mice were pretreated with aspirin (150 μmol/kg), NOSH-ASA (150 μmol/kg) or vehicle orally 30 min before the intraplantar injection of carrageenan (Cg; 100 μg/paw) or saline. At 3 h after Cg injection plantar tissue were collected for measurement of (A) neutrophil migration (MPO activity assay) and the levels of (B) TNF-α, (C) KC/CXCL1, and (D) IL1-β by ELISA. Data are the means ± SEM (n = 6). ***P < 0.001 versus saline group. ^#P < 0.05 versus vehicle-treated group. MPO, myeloperoxidase; TNF-α, tumor necrosis factor-α; KC/CXCL1, keratinocyte-derived chemokine.