Figure 6.

Effect of NOSH-aspirin (NOSH-ASA) and aspirin on PGE₂-induced hyperalgesia: involvement of K_ATP. (A) Mice were pretreated 50 min before with aspirin (150 μmol/kg), NOSH-ASA (150 μmol/kg) or vehicle (v) orally followed by intraplantar (i.p.) injection of PGE₂. Mechanical hyperalgesia was evaluated 60 min after PGE₂ injection. (B) Mice were pretreated with K_ATP blocker (glibenclamide; 10 mg/kg, i.p.) and after 30 min, they received vehicle or NOSH-ASA (150 μmol/kg), and after 50 min all groups received i.p. injection of PGE₂. Mechanical hyperalgesia was evaluated 60 min after PGE₂ injection. Data are the means ± SEM (n = 6). **P < 0.01 and ***P < 0.001 versus vehicle group, ^#P < 0.001 versus NOSH-ASA-treated group. K_ATP, ATP-sensitive potassium channel; PGE₂, prostaglandin E₂.