Table 2.
Mean (±SEM) plasma biochemical parameters and organ weights in mice.
| Sham + Veh | EAE + Veh | EAE + ALA (10 mg kg−1 day−1) | |
|---|---|---|---|
| Biochemical parameters (mean ± SEM) | |||
| ALT (U/L) | 29.25 ± 4.6 | 24.75 ± 9.1 | 27.5 ± 5.9 |
| AST (U/L) | 394.8 ± 53.3 | 324.5 ± 73.3 | 313.0 ± 85.7 |
| ALP (U/L) | 31.0 ± 10.9 | 38.8 ± 9.9 | 38.8 ± 3.3 |
| Total bilirubin, μmol/L | 2.5 ± 0.5 | 6.3 ± 2.5 | 2.0 ± 0.0 |
| Urea mmol/L | 4.4 ± 0.2 | 3.7 ± 0.5 | 4.6 ± 0.1 |
| Creatinine*, μmol/L | BLOQ | BLOQ | BLOQ |
| Organ weights (mg; mean ± SEM) | |||
| Body weights (at euthanasia) | 20.2 ± 0.4 | 19.6 ± 0.3 | 19.5 ± 0.3 |
| Liver | |||
| Absolute | 947 ± 40.0 | 925 ± 35.9 | 894.5 ± 37.9 |
| Relative | 4.68 ± 0.14 | 4.71 ± 0.15 | 4.57 ± 0.19 |
| Kidneys | |||
| Absolute | 247.6 ± 10.1 | 262.1 ± 8.1 | 260.1 ± 10.0 |
| Relative | 1.22 ± 0.03 | 1.33 ± 0.02 | 1.33 ± 0.05 |
ALA, alpha lipoic acid; ALT, alanine transaminase; AST, aspartate transaminase; ALP, alkaline phosphatise; EAE, experimental autoimmune encephalomyelitis; SEM, standard error mean; Veh, vehicle.
*
Below lower limit of quantification (BLOQ) which was <20 μmol/L; the normal range for C57BL6 mice is ≤20 μmol/L (Zhou and Hansson 2004).