Table 3.
Choline-induced Isc is carried by Cl− secretion
| Conditions | Choline-induced increase in Isc (ΔIsc; μEq/h per cm2) | n |
|---|---|---|
| With chloride | 1.25 ± 0.30 | 7 |
| Without chloride | 0.30 ± 0.19* | 8 |
| Without bumetanide | 0.64 ± 0.18 | 7 |
| With bumetanide | 0.15 ± 0.03* | 8 |
Chloride dependency of the Isc response evoked by choline (7.5 × 10−3 mol/L) across mucosa–submucosa preparations from rat distal colon. For each experimental series, the response to choline was tested in the presence of bumetanide (10−4 mol/L) or after chloride substitution (at the mucosal and serosal side), respectively, and compared to the response under control conditions. Values are given as increase in Isc above baseline just prior to administration of choline (ΔIsc) and are means ± SEM.
*
P < 0.05 vs. response evoked by choline in the respective control series.