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. 2015 Jul 6;10(7):e0132032. doi: 10.1371/journal.pone.0132032

Fig 1. Analysis of LPS expression and immunoreactivity.

Fig 1

(A) LPS extracted from B. mallei (Lane 2), SL3261 (Lane 3), and SL3261/p1C3 (Lane 4) was subjected to SDS-PAGE followed by immunoblotting analysis using 5C8-1C3 anti-B. mallei-specific LPS monoclonal antibody (mAb). Molecular weight marker is shown in Lane 1. (B) LPS extracted from B. mallei (B.m.) and B. pseudomallei (B.p) was run, subjected to SDS-PAGE followed by immunoblot using sera collected from SL3261 (vector)-immunized mice; 3B3-5, anti-B. pseudomallei mAb; sera collected from SL3261/p1C3 (vaccine)-immunized mice; and 5C8-1C3, anti-B. mallei mAb. Molecular weight ladder (L).