EGFR and HER2 FISH. Images of metaphase and interphase nuclei after FISH are presented. The EGFR and HER2 genes are localized by red fluorescent signals, and chromosome 7 and 17 centromeres (CEP7 and CEP17) are localized by green fluorescent signals. The cells were counterstained with DAPI (blue). EGFR/CEP7 staining of a SCC58 and b SCC28 cells. HER2/CEP17 staining of c SCC25, d HN5, e SCC58, f SQ20B, g SCC61, and h SCC28