Table 2.
EGFR and HER2 gene copy number alterations and mRNA expression levels of SCCHN cell lines
| Cell line | FISH | qPCR | ||||||
|---|---|---|---|---|---|---|---|---|
| EGFR GCN1 | CEP7 GCN2 | EGFR/CEP7 3 | Interpretation | TGFa/18 s | AREG/18 s | EGFR/18 s | Interpretation | |
| SCC58 | 32.94 | 4.3 | 7.7 | High Ampl | 2.02 | 9.67 | 17.08 | Gain |
| SCC28 | 5.02 | 5.33 | 0.94 | No Ampl, | 0.39 | 1.86 | 1.77 | Normal |
| HER2 GCN1 | CEP17 GCN2 | HER2/CEP17 3 | Interpretation | |||||
| SCC25 | 3.25 | 3.05 | 1.07 | No Ampl, | ||||
| HN5 | 2.88 | 3.9 | 0.74 | No Ampl, | ||||
| SCC58 | 1.87 | 2.87 | 0.65 | No Ampl | ||||
| SQ20B | 2.06 | 4.20 | 0.49 | No Ampl | ||||
| SCC61 | 2.3 | 2.04 | 1.13 | No Ampl | ||||
| SCC28 | 2.85 | 2.93 | 0.97 | No Ampl, | ||||
FISH: 1Mean copy number EGFR or HER2 per cell; 2mean copy number of centromere enumeration probe (CEP7 or CEP17) per cell; 3mean gene to centromere ratio; Ampl, amplification, ≥15 copies of gene per cell in ≥10 % of cells or gene to CEP ratio ≥2.0. An average of 80 (range, 50 to 180) well-defined malignant nuclei were scored
qPCR: Mean values from three experiments were calculated relative to 18 s as control, arbitrarily set at 1. Gain, ≥7 copies of gene/18 s